The Clinical Pathology Lab offers the following coagulation tests with the listed protocols.
Customized pricing may apply for high volume testing, batch testing, or panels of tests. Please contact the Clinical Pathology Lab by phone, 612-625-1746, or by email, [email protected], for additional information regarding discount pricing.
Coagulation tests
| Research, Coagulation | Test list | Specimen | Minimum volume (plasma) | Species |
|---|---|---|---|---|
| Protime (PT)/Activated Partial Thromboplastin Time (aPTT)/Quantitative Fibrinogen Assy (QFA) | Prothrombin Time Activated Partial Thromboplastin Time Fibrin Split Products Platelet Count Fibrinogen | Sodium Citrate (3.2%) plasma & EDTA whole blood | 2.0 ml | Feline Canine Equine Bovine Ovine |
| Prothrombin Time (PT) | Prothrombin Time | Sodium Citrate (3.2%) plasma | 2.0 ml | Feline Canine Equine Bovine Ovine |
| Activated Partial Thromboplastin Time (aPTT) | Activated Partial Thromboplastin Time | Sodium Citrate (3.2%) plasma | 2.0 ml | Feline Canine Equine Bovine Ovine |
| PT, aPTT | Prothrombin Time Activated Partial Thromboplastin Time | Sodium Citrate (3.2%) plasma | 2.0 ml | Feline Canine Equine Bovine Ovine |
| Quantitative Fibrinogen Assay (QFA) | Quantitative Fibrinogen Assay | Sodium Citrate (3.2%) plasma | 2.0 ml | Feline Canine Equine Bovine |
Coagulation protocols
Collection protocol
Instruments
IL ACL TOP300; Werfern USA, LLC, Pittsburgh, PA
Sample Requirements
Citrated plasma (light blue top tube); tubes of appropriate size should be selected to ensure an optimal ratio of citrate to whole blood. Required volumes are listed in the table below. Tubes should be centrifuged at 2500 rcf* for 10 minutes, citrated plasma removed with a plastic pipet tip and placed into a sterile, non-citrated, plastic tube within 2 hours of collection. Samples must not come into contact with glass. Samples will be run the same day, and samples >24 hours old will be rejected. Please contact the lab for further instruction if you would like to submit archived, frozen samples for analysis.
(*rcf = relative centrifugal force = 0.00001118 x r (rotational radius in cm) x RPM2)
| Option | Description of item | Required 3.2% NaCit plasma volume (Note: not whole blood volume) |
|---|---|---|
| 1 | Prothrombin Time (PT) | 250 µLs** |
| 2 | Activated Partial Thromboplastin Time (aPTT) | 250 µLs** |
| 3 | PT and aPTT | 500 µLs** |
| 4 | Protime (PT)/Activated Partial Thromboplastin Time (aPTT)/Quantitative Fibrinogen Assy (QFA) | 500 µLs** - citrated plasma |
**If the specimen is short volume, the lab will attempt to run as many tests as possible.
Appropriate Tubes
The following tubes are suitable for blood collection and analysis using the STA Compact and FDP Plasma Kit. Please contact the lab if you have questions regarding the appropriate tube to utilize for your samples.
| Name | Sample volume | Mfr # |
|---|---|---|
| BD Vacutainer Blood Tubes with 3.2% sodium citrate | 1.8 mL | 363080 |
| BD Vacutainer Blood Tubes with 3.2% sodium citrate | 2.7 mL | 363083 |
Sample handling
Following collection, tubes should be inverted 8 times to ensure adequate mixing of blood and sodium citrate. Label tubes using permanent marker with a legible, simplified numbering system. Samples should be refrigerated as soon as possible following collection. They should not be frozen or transported directly on ice. Transport with cold packs is recommended.
Results
Results are available in Excel format.
Common Interferences
Complete filling of the tube is important to ensure the appropriate ratio of citrate to whole blood (1:9 or 3.2% citrate). Over-citrated plasma (under-filled tube) may result in prolonged clotting times. Under-citrated plasma (over-filled tube) may result in shortened clotting times.