Chemistry Tests and Protocols

Chemistry Single Tests
Chemistry Panels
Small Volume Protocols
Large Volume Protocols

Chemistry Single Tests

Serum

Test name	Test initials	Specimen	Minimum volume
Alanine Aminotransferase	ALT	Serum	50 ul
Albumin	Alb	Serum	50 ul
Alkaline Phosphatase	ALP	Serum	50 ul
Amylase	Amy	Serum	50 ul
Aspartate Aminotransferase	AST	Serum	50 ul
Beta-hydroxybutyrate	BHY	Serum	50 ul
Bicarbonate	HCO3	Serum	50 ul
Bile acid (Fasting & Post Prandial)	Bile	Serum	50 ul
Bilirubin, Total	Tbili	Serum	50 ul
Blood Urea Nitrogen	BUN	Serum	50 ul
Calcium	Ca	Serum	50 ul
Chloride	Cl	Serum	50 ul
Cholesterol	Chol	Serum	50 ul
Creatine Kinase	CK	Serum	50 ul
Creatinine	Creat	Serum	50 ul
Gamma Glutamyltransferase	GGT	Serum	50 ul
Glucose	Gluc	Serum	50 ul
Lactate Dehydrogenase	LDH	Serum	50 ul
Magnesium	Mg	Serum	50 ul
Phenobarbitol	Pheno	Serum	50 ul
Phosphorous	Phos	Serum	50 ul
Potassium	K	Serum	50 ul
Sodium	Na	Serum	50 ul
Sorbitol Dehydrogenase	SDH	Serum	50 ul
Total Protein	TP	Serum	50 ul
Triglyceride	Trig	Serum	50 ul

CSF

Test name	Test initials	Specimen	Minimum Volume
CSF Creatine Kinase	CKCSF	Spinal Fluid	50 ul
CSF Glucose	GluCSF	Spinal Fluid	50 ul
CSF Total Protein	MTPCSF	Spinal Fluid	50 ul
CSF CK & Protein		Spinal Fluid	75 ul
CSF Protein, CK and Glucose		Spinal Fluid	75 ul

Urine

Test name	Test initials	Specimen	Minimum Volume
Urine Calcium	CaU	Urine	50 ul
Urine Chloride	ClU	Urine	50 ul
Urine Creatinine	CreatU	Urine	50 ul
Urine Chloride/Creatinine	Cl/CreatU	Urine	75 ul
Urine Gamma Glutamyltransferase	GGTU	Urine	50 ul
Urine Magnesium	MgU	Urine	50 ul
Urea Nitrogen	Urea	Urine	50 ul
Urine Phosphorous	PhosU	Urine	50 ul
Urine Potassium	KU	Urine	50 ul
Urinary Protein	MTPU	Urine	50 ul
Urine Protein/Creatinine Ratio	UP/UC	Urine	50 ul
Urine Sodium	NaU	Urine	50 ul

Chemistry Panels

Research Panels Available	Tests included in panels	Minimum Volume	Species
Electrolyte Profile	Anion Gap, HCO3, Ca, Cl, Mg, Phos, Na, K, LIH	250 ul	Alpacas, Bovine, Camelids, Canines, Equine, Feline, Llamas, Mice, Mink, Porcine, Rabbits, Rats, Other
Large Animal Hepatic	Alb, ALP, AST, ALT, CK, GGT, SDH, TP, Gluc, Tbili, LIH	250 ul	Alpacas, Bovine, Camelids, Equine, Llamas, Porcine, Other
Large Animal Mini-Basic	HCO3, Ca, Cl, CK, GGT, Gluc, K, TP, Creat, Na, LIH	250 ul	Alpacas, Bovine, Camelids, Equine, Llamas, Porcine, Other
Large Animal Profile	Alb, ALP, Anion Gap, AST, HCO3, Tbili, BUN, Ca, Cl, CK, Creat, GGT, Gluc, Glob, LIH, MG, Osmolality, Phos, K, Na, SDH, TP	250 ul	Alpacas, Bovine, Camelids, Equine, Llamas, Porcine, Other
Non-human Primate	ALT, Alb, ALP, Anion Gap, AST, HCO3, Tbili, BUN, Ca, Cl, Chol, CK, Creat, GGT, Globulin, Gluc, LIH, Mg, Osmolality, K, Na, TP, Trig	250 ul	Non-human Primates
Pick 2 Research Panel	Pick any two analytes from the single test list (Excludes Phenobarbitol, Trig, LDH & Bile Acids)	50 ul	Serum Samples ONLY. Available for most lab species. Check with lab for exotic species.
Pick 5 Research Panel	Pick any five analytes from the single test list (Excludes Phenobarbitol, Trig, LDH & Bile Acids)	100 ul	Serum Samples ONLY. Available for most lab species. Check with lab for exotic species.
Renal Profile	Alb, Anion Gap, BUN, Ca, Chol, Cl, Creat, HCO3, Gluc,K, Mg, Na, Osmolality,Total Protein, BHY, Phos	250 ul	Available for routinely utilized lab species. Check with lab for exotic species.
Small Animal Hepatic	ALT, Alb, ALP, Amylase, AST. Tbili, Chol, GGT, TP	250 ul	Canine, Feline, Mice, Mink, Rabbits, Rats
Small Animal Profile	ALT, Alb, ALP, Amylase, Anion Gap, AST, HCO3, BUN, Ca, Cl, Chol, Creat, GGT Gluc,Globulin, LIH, Mg, Osmolality, Phos, K, Na, TP, Tbili, CK	250 ul	Canine, Feline, Mice, Mink, Rabbits, Rats
Small Animal, Basic	ALT, Alb, ALP, Tbili, BUN, Creat, Gluc, K, Na, TP, LIH, Anion Gap, C1, HCO3	250 ul	Canine, Feline, Mice, Mink, Rabbits, Rats
Urine Profile	Cl, Creat, K, Na	150 ul	Most species. Check with lab for exotic species.

Small Volume Protocols

Small volume biochemical profile: Collection protocols for small volume specimens (mice, rats, rabbits, etc.)

Instrument

Beckman Coulter AU480; Beckman Coulter, Brea, CA

Sample Requirements

Blood should be collected into plain red top tubes with no additive or gel separator. Following collection, blood should be allowed to clot, centrifuged at 1700 rcf* for 10 minutes, the serum removed and placed into another plain red top tube or other, sterile, additive-free, plastic tube. To prevent analytical error, serum should be removed from the clot within 1 hour of sampling. Required volumes are listed in the table below.

*RCF = relative centrifugal force = 0.00001118 x r (rotational radius in cm) x RPM2

Option	Description of item	Required serum volume (Note: not whole blood volume)
1	Single test	50 μLs**
2	Two tests	75 μLs**
3	Research panel of up to 5 tests (can be customized)	100 μLs**
4	Larger panels and profiles	250 μLs**

**If the specimen is short volume, the lab will attempt to run as many tests as possible.

Appropriate Tubes

The following tubes are suitable for blood collection and analysis using the Beckman AU480. Please contact the lab if you have questions regarding the appropriate tube to utilize for your samples.

Name	Sample volume	Mfr #
BD Microtainer Capillary Blood Collector	400 uL	365957
BD Microtainer Capillary Blood Collector with BD Microgard Closure	250-500 uL	365963

Sample Handling

Label tubes using permanent marker with a legible, simplified numbering system. Samples should be refrigerated as soon as possible following collection. Transport with cold packs is recommended; do not transport directly on ice. Please contact the lab for further instruction if you would like to submit archived, frozen samples for analysis.

Results

Results are available in Excel format.

Common Interferences

Certain degrees of lipemia, hemolysis, and icterus (LIH) may interfere with analysis of some analytes. LIH indices and the expected interferences are reported for basic and full profile samples. Potassium levels may be artifactually increased and glucose artifactually decreased in samples where serum has sat on the blood clot for an extended period of time.

Large Volume Protocols

Large volume biochemical profile: Collection protocols for large volume specimens (dogs, pigs, camelids, horses, etc.)