Chemistry Tests and Protocols
Chemistry Single Tests
Serum
Test name | Test initials | Specimen | Minimum volume |
Alanine Aminotransferase | ALT | Serum | 50 ul |
Albumin | Alb | Serum | 50 ul |
Alkaline Phosphatase | ALP | Serum | 50 ul |
Amylase | Amy | Serum | 50 ul |
Aspartate Aminotransferase | AST | Serum | 50 ul |
Beta-hydroxybutyrate | BHY | Serum | 50 ul |
Bicarbonate | HCO3 | Serum | 50 ul |
Bile acid (Fasting & Post Prandial) | Bile | Serum | 50 ul |
Bilirubin, Total | Tbili | Serum | 50 ul |
Blood Urea Nitrogen | BUN | Serum | 50 ul |
Calcium | Ca | Serum | 50 ul |
Chloride | Cl | Serum | 50 ul |
Cholesterol | Chol | Serum | 50 ul |
Creatine Kinase | CK | Serum | 50 ul |
Creatinine | Creat | Serum | 50 ul |
Gamma Glutamyltransferase | GGT | Serum | 50 ul |
Glucose | Gluc | Serum | 50 ul |
Lactate Dehydrogenase | LDH | Serum | 50 ul |
Magnesium | Mg | Serum | 50 ul |
Phenobarbitol | Pheno | Serum | 50 ul |
Phosphorous | Phos | Serum | 50 ul |
Potassium | K | Serum | 50 ul |
Sodium | Na | Serum | 50 ul |
Sorbitol Dehydrogenase | SDH | Serum | 50 ul |
Total Protein | TP | Serum | 50 ul |
Triglyceride | Trig | Serum | 50 ul |
CSF
Test name | Test initials | Specimen | Minimum Volume |
CSF Creatine Kinase | CKCSF | Spinal Fluid | 50 ul |
CSF Glucose | GluCSF | Spinal Fluid | 50 ul |
CSF Total Protein | MTPCSF | Spinal Fluid | 50 ul |
CSF CK & Protein | Spinal Fluid | 75 ul | |
CSF Protein, CK and Glucose | Spinal Fluid | 75 ul |
Urine
Test name | Test initials | Specimen | Minimum Volume |
Urine Calcium | CaU | Urine | 50 ul |
Urine Chloride | ClU | Urine | 50 ul |
Urine Creatinine | CreatU | Urine | 50 ul |
Urine Chloride/Creatinine | Cl/CreatU | Urine | 75 ul |
Urine Gamma Glutamyltransferase | GGTU | Urine | 50 ul |
Urine Magnesium | MgU | Urine | 50 ul |
Urea Nitrogen | Urea | Urine | 50 ul |
Urine Phosphorous | PhosU | Urine | 50 ul |
Urine Potassium | KU | Urine | 50 ul |
Urinary Protein | MTPU | Urine | 50 ul |
Urine Protein/Creatinine Ratio | UP/UC | Urine | 50 ul |
Urine Sodium | NaU | Urine | 50 ul |
Chemistry Panels
Research Panels Available | Tests included in panels | Minimum Volume | Species |
Electrolyte Profile | Anion Gap, HCO3, Ca, Cl, Mg, Phos, Na, K, LIH | 250 ul | Alpacas, Bovine, Camelids, Canines, Equine, Feline, Llamas, Mice, Mink, Porcine, Rabbits, Rats, Other |
Large Animal Hepatic | Alb, ALP, AST, ALT, CK, GGT, SDH, TP, Gluc, Tbili, LIH | 250 ul | Alpacas, Bovine, Camelids, Equine, Llamas, Porcine, Other |
Large Animal Mini-Basic | HCO3, Ca, Cl, CK, GGT, Gluc, K, TP, Creat, Na, LIH | 250 ul | Alpacas, Bovine, Camelids, Equine, Llamas, Porcine, Other |
Large Animal Profile | Alb, ALP, Anion Gap, AST, HCO3, Tbili, BUN, Ca, Cl, CK, Creat, GGT, Gluc, Glob, LIH, MG, Osmolality, Phos, K, Na, SDH, TP | 250 ul | Alpacas, Bovine, Camelids, Equine, Llamas, Porcine, Other |
Non-human Primate | ALT, Alb, ALP, Anion Gap, AST, HCO3, Tbili, BUN, Ca, Cl, Chol, CK, Creat, GGT, Globulin, Gluc, LIH, Mg, Osmolality, K, Na, TP, Trig | 250 ul | Non-human Primates |
Pick 2 Research Panel | Pick any two analytes from the single test list (Excludes Phenobarbitol, Trig, LDH & Bile Acids) | 50 ul | Serum Samples ONLY. Available for most lab species. Check with lab for exotic species. |
Pick 5 Research Panel | Pick any five analytes from the single test list (Excludes Phenobarbitol, Trig, LDH & Bile Acids) | 100 ul | Serum Samples ONLY. Available for most lab species. Check with lab for exotic species. |
Renal Profile | Alb, Anion Gap, BUN, Ca, Chol, Cl, Creat, HCO3, Gluc,K, Mg, Na, Osmolality,Total Protein, BHY, Phos | 250 ul | Available for routinely utilized lab species. Check with lab for exotic species. |
Small Animal Hepatic | ALT, Alb, ALP, Amylase, AST. Tbili, Chol, GGT, TP | 250 ul | Canine, Feline, Mice, Mink, Rabbits, Rats |
Small Animal Profile | ALT, Alb, ALP, Amylase, Anion Gap, AST, HCO3, BUN, Ca, Cl, Chol, Creat, GGT Gluc,Globulin, LIH, Mg, Osmolality, Phos, K, Na, TP, Tbili, CK | 250 ul | Canine, Feline, Mice, Mink, Rabbits, Rats |
Small Animal, Basic | ALT, Alb, ALP, Tbili, BUN, Creat, Gluc, K, Na, TP, LIH, Anion Gap, C1, HCO3 | 250 ul | Canine, Feline, Mice, Mink, Rabbits, Rats |
Urine Profile | Cl, Creat, K, Na | 150 ul | Most species. Check with lab for exotic species. |
Small Volume Protocols
Small volume biochemical profile: Collection protocols for small volume specimens (mice, rats, rabbits, etc.)
Instrument
Beckman Coulter AU480; Beckman Coulter, Brea, CA
Sample Requirements
Blood should be collected into plain red top tubes with no additive or gel separator. Following collection, blood should be allowed to clot, centrifuged at 1700 rcf* for 10 minutes, the serum removed and placed into another plain red top tube or other, sterile, additive-free, plastic tube. To prevent analytical error, serum should be removed from the clot within 1 hour of sampling. Required volumes are listed in the table below.
*RCF = relative centrifugal force = 0.00001118 x r (rotational radius in cm) x RPM2
Option | Description of item |
Required serum volume |
1 | Single test | 50 μLs** |
2 | Two tests | 75 μLs** |
3 | Research panel of up to 5 tests (can be customized) | 100 μLs** |
4 | Larger panels and profiles | 250 μLs** |
**If the specimen is short volume, the lab will attempt to run as many tests as possible.
Appropriate Tubes
The following tubes are suitable for blood collection and analysis using the Beckman AU480. Please contact the lab if you have questions regarding the appropriate tube to utilize for your samples.
Name | Sample volume | Mfr # |
BD Microtainer Capillary Blood Collector | 400 uL | 365957 |
BD Microtainer Capillary Blood Collector with BD Microgard Closure | 250-500 uL | 365963 |
Sample Handling
Label tubes using permanent marker with a legible, simplified numbering system. Samples should be refrigerated as soon as possible following collection. Transport with cold packs is recommended; do not transport directly on ice. Please contact the lab for further instruction if you would like to submit archived, frozen samples for analysis.
Results
Results are available in Excel format.
Common Interferences
Certain degrees of lipemia, hemolysis, and icterus (LIH) may interfere with analysis of some analytes. LIH indices and the expected interferences are reported for basic and full profile samples. Potassium levels may be artifactually increased and glucose artifactually decreased in samples where serum has sat on the blood clot for an extended period of time.
Large Volume Protocols
Large volume biochemical profile: Collection protocols for large volume specimens (dogs, pigs, camelids, horses, etc.)
Instrument
Beckman AU480, Beckman Coulter, Brea, CA
Sample Requirements
Blood should be collected into plain red top tubes with no additive or gel separator. Following collection, blood should be allowed to clot, centrifuged at 1700 rcf* for 10 minutes, the serum removed and placed into another plain red top tube or other, sterile, additive-free, plastic tube. To prevent analytical error, serum should be removed from the clot within 1 hour of sampling. Required volumes are listed in the table below.
*RCF = relative centrifugal force = 0.00001118 x r (rotational radius in cm) x RPM2
Option | Description of item |
Required serum volume |
1 | Single test | 50 μLs** |
2 | Two tests | 75 μLs** |
3 | Research panel of up to 5 tests (can be customized) | 100 μLs** |
4 | Larger panels and profiles | 250 μLs** |
**If the specimen is short volume, the lab will attempt to run as many tests as possible.
Appropriate Tubes
The following tubes are suitable for blood collection and analysis using the Beckman Coulter AU480. Please contact the lab if you have questions regarding the appropriate tube to utilize for your samples.
Name | Sample volume | Mfr # |
BD Vacutainer Blood Tubes (glass tubes, no additive) | 3 mL | 366397 |
BD Vacutainer Blood Tubes (glass tubes, no additive) | 7 mL | 366397 |
BD Vacutainer Blood Tubes (glass tubes, no additive) | 10 mL |
Sample Handling
Label tubes using permanent marker with a legible, simplified numbering system. Samples should be refrigerated as soon as possible following collection. Transport with cold packs is recommended; do not transport directly on ice. Please contact the lab for further instruction if you would like to submit archived, frozen samples for analysis.
Results
Results are available in Excel format.
Common Interferences
Certain degrees of lipemia, hemolysis, and icterus (LIH) may interfere with analysis of some analytes. LIH indices and the expected interferences are reported for basic and full profile samples. Potassium levels may be artifactually increased and glucose artifactually decreased in samples where serum has sat on the blood clot for an extended period of time.